Belfiore, A., R. P. Buley, E. G. Fernandez-Figueroa, M. Gladfelter, and A. E. Wilson. 2021. Zooplankton as an alternative method for controlling phytoplankton in catfish pond aquaculture. Aquaculture Reports 21:100897.
In pond aquaculture, production of toxins and off-flavor compounds by cyanobacteria can negatively affect fish health and production. Studies have explored chemical or physical methods for controlling algal blooms in aquaculture ponds, which although effective, may be short-lived and can negatively impact non-target organisms, including aquaculture species. Food web manipulations have a long history in lake and fisheries management to improve water quality, but have been rarely considered in aquaculture. This study examined zooplankton and phytoplankton communities, cyanobacterial toxins, and nutrients in nine catfish aquaculture farm-ponds in west Alabama, USA. The goal of this project was to track phytoplankton and zooplankton abundances with respect to each other, with and without efforts to reduce zooplanktivorous fish in some of the ponds. During this project, farm managers reduced zooplanktivorous fish abundance in select ponds to create a large-scale field experiment that addressed the role of zooplankton control of phytoplankton in hypereutrophic catfish aquaculture ponds when zooplanktivorous fish were or were not excluded. There was a strong negative effect of zooplankton on phytoplankton, including cyanobacteria, despite high nutrient concentrations. Although high zooplankton ponds sustained elevated zooplankton biomass during much of this study, including when pond temperatures exceeded 30 °C, the effect of zooplankton on phytoplankton was most pronounced during the non-growing season (November–April). In addition, total ammonia nitrogen was significantly higher in high zooplankton ponds, which could lead to ammonia toxicity in fish at elevated temperature and pH. Our findings suggest that zooplankton biomanipulation may be an efficient method to control algal blooms in farm-pond catfish aquaculture.
Berry, J. P., E. Lee, K. Walton, A. E. Wilson, and F. Bernal-Brooks. 2011. Microcystin production by a persistent cyanobacterial bloom in Lago de Patzcuaro (Michiacan, Mexico) and apparent bioaccumulation of the toxin in small commercial catches of fish. Environmental Toxicology and Chemistry 30(7):1621-1628.
Lago de Patzcuaro is a historically important freshwater fishery in Mexico. The lake is presently characterized by a persistent bloom of cyanobacteria, specifically dominated by recognized producers of toxic microcystins (MCYSTs). We evaluated MCYSTs in sestonic and dissolved fractions of the water column, as well as representative fish species (silversides, Chirostoma spp.; Goodea sp.; and carp, Cyprinus carpio) obtained from local markets and small commercial catches during the bloom. Samples were evaluated primarily by enzyme-linked immunosorbent assay (ELISA), and secondarily by protein phosphatase (PPase) inhibition assay and liquid chromatography-mass spectrometry (LC-MS). Sestonic MCYST concentration (0.02-0.36 µg/L) generally correlated inversely with distance from the bloom, supporting the bloom as the source of the toxin. Several MCYST variants, including MC-LR, -LA and -LY, as well as didemethyl variants, were identified by LC-MS/MS analysis. All three species of fish bioaccumulated MCYSTs in relevant tissues, and toxin content correlated with trophic level, with highest and lowest levels measured in phytoplanktivorous and zooplanktivorous representatives, respectively. Detection of MCYST in silversides and Goodea sp. is particularly relevant because both are consumed in their entirety, including viscera (e.g., liver) known to primarily accumulate MCYST. These results indicate that Lago de Patzcuaro is indeed characterized by a toxigenic bloom, and that commercially important fish species from the lake accumulate toxic MCYST in tissues relevant to human consumption. As such, this system may represent an ideal model of the trophic transfer of MCYSTs and its relevance to human and environmental health.